Regulatory

Part:BBa_K779409

Designed by: Divya Israni   Group: iGEM12_MIT   (2012-09-29)

U6-TetO-Sc MammoBlock

The U6-TetO-Sc part encodes conditional expression of the short RNA sequence Sc. The mammalian RNA Pol III U6 promoter incorporates tetracycline-responsive elements that allow for tightly regulated RNA expression under specific conditions. In the presence of the Tet repressor (TetR), two Tet operator (TetO) sites become bound by the TetR and prevent RNA Pol III from binding, subsequently inhibiting transcription of the downstream sequence. In the absence of TetR, RNA transcription proceeds normally. The expression of the short Sc RNA sequence is to be used for in vivo RNA strand displacement of a downstream gate-output complex.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aU6-TetO-Sc MammoBlock